The apoptotic effect induced by cafestol and kahweol in MSTO-211H cells. Cells were incubated with cafestol (30, 60, and 90 μM) and kahweol (20, 40, and 60 μM) and untreated (DMSO) for 48 hours. The cells were harvested and prepared for DAPI staining and PI staining as described in the Methods section. (A) Analysis of DNA fragmentation and nuclear condensation by fluorescence microscopy (magnification X600) after cafestol and kahweol treatement in MSTO-211H cells. (B) DNA fragmentation and nuclear condensation were quantified, and the results in triplicates are expressed as the mean ± SD. Analysis of cell cycle by flow cytometry after cafestol (C) and kahweol (D) treatment of cells for 48 hours. Representative of Sub-G1 population. The cafestol or kahweol-treated cells were compared with untreated cells, and data are shown as the average of triplicate samples from three independent experiments. The asterisks (*) indicates p < 0.05 versus control cells.