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Figure 6 | Journal of Biomedical Science

Figure 6

From: Natural diterpenes from coffee, cafestol and kahweol induce apoptosis through regulation of specificity protein 1 expression in human malignant pleural mesothelioma

Figure 6

The effect of cafestol and kahweol on apoptosis of MSTO-211H cells. Fifty micrograms of cellular extract per lane were separated on an SDS-PAGE gel as described in materials and methods. Immunoblot detection of Bid, Caspase-3, and PARP, upregulation of Bax, downregulation of Bcl-xl in whole cell lysates from various concentration of cafestol and kahweol-treated MSTO-211H cells for 48 hours. Equal loading of protein was confirmed by incubating the same membrane with anti-β-Actin antibody.

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Journal of Biomedical Science

ISSN: 1423-0127