Figure 4From: Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cellsGO treatment induces autophagy. Cells expressing GFP-LC3 generated using the controls (upper half) and cells overexpressing IRS-1 (lower half) were treated with 10 mU/ml GO or vehicle (DMSO) for 6 h, and observed under a fluorescence microscope.Back to article page