Figure 5From: Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cellsGO treatment induces autophagy. Control cells (upper half) and cells overexpressing IRS-1 (lower half) were treated with 10 mU/ml GO or vehicle (DMSO) for 6 h, stained with anti-LC3B antibody and goat anti-rabbit IgG conjugated with Alexa488, and observed under a fluorescence microscope.Back to article page