Figure 7

Effect of IRS-1 on oxidative stress-mediated cell fate. (A) Control cells and cells overexpressing IRS-1 were treated with 5 mU/ml GO for 9 h. Cell proliferation was measured using alamarBlue assay. (B) Wild-type NIH/3T3 cells were treated with 10 mU/ml GO for 24 h. Dead cells were observed using an electron microscope. The scale bar in panel B-2 represents 2 μm, and in B-b, represents 500 nm. Black arrowheads indicate autophagic vacuoles. (C) Control cells and cells overexpressing IRS-1 were treated with 5 or 10 mU/ml GO for 6 h. Cells were collected by trypsinization and stained with trypan blue. Dead cells were observed and counted under a light microscope. Three dishes of cells of each condition were observed for statistical analysis. Results from a representative of three independent experiments are shown. (D) Control cells and cells overexpressing IRS-1 were treated with 10 mU/ml GO for 6 h. Cells were collected by trypsinization and stained with PI. Cell death was analyzed from the proportion of sub-G1 group DNA determined by flow cytometry analysis. Four independent experiments were performed for statistical analysis. M1 indicates the sub-G1 DNA area.