CLSM micrographs of apoptosis and/or necrosis in malignant melanoma cell line (MM96L). Cells were incubated for 3 h with 100 ng/mL, 200 ng/mL or 400 ng/mL of RRM-MV or RRM-C in (A); or with 100 ng/mL of RRM-MV or RRM-C for 18 h in (B). After incubation, cells were double stained with AF488 and PI. CLSM images show that RRM-MV induced cell apoptosis/necrosis effects. Prolonged incubation with RRM-MV caused stronger cytotoxic effects. AF488 channels show apoptotic cells labeled with green-fluorescent, and PI channels show necrotic cells labeled with red-fluorescent. CLSM images were taken with Nikon Eclipse Ti-E A1 laser-scanning confocal system, and analysed with the NIS-Element imaging software (100x magnification).