Figure 4
p-Akt, endothelial nitric oxide synthase, nitrite/nitrate and HIF1-α. A: Representative western blotting of p-Akt (upper panels) and densitometric analysis (lower panels); B: Representative western blotting of endothelial nitric oxide synthase (eNOS) (upper panels) and densitometric analysis (lower panels); C: Nitrite/nitrate levels; D: Hypoxia-induced factor-1α (HIF-1α) protein levels. Sham group: rats underwent only dissection; I/R group: renal pedicles underwent 60 min of warm ischemia (I) followed by 120 min of reperfusion (R). IPC group: renal pedicles underwent 2 cyles of 5 min of I and 5 min of R, just before sustained I. L-NAME+IPC group: 5 min before IPC, 5 mg/kg of NG-nitro-L-arginine methylester (L-NAME) was intravenously administered. Results are presented as mean ± SEM (n = 6 in each group). *P < 0.05 vs. Sham group, #P < 0.05 vs. I/R group, +P < 0.05 vs. IPC group.