Figure 4
From: Regulation of PUMA induced by mechanical stress in rat cardiomyocytes
Effects of cyclic stretch on PUMA expression in cardiomyocytes. (A) Representative Western blots for PUMA in cardiomyocytes subjected to cyclic stretch by 20 or 10 % for various periods of time. (B) Quantitative analysis of PUMA protein levels. The values from stretched cardiomyocytes have been normalized to matched α-tubulin measurement and then expressed as a ratio of normalized values to protein in control group (n = 4 per group). *P < 0.01 vs. control. (C) Quantitative analysis of PUMA mRNA levels. The values from stretched cardiomyocytes have been normalized to matched GAPDH measurement and then expressed as a ratio of normalized values to mRNA in control group (n = 5 per group). *P < 0.01 vs. control.