Figure 5From: Regulation of PUMA induced by mechanical stress in rat cardiomyocytesEffects of MAP kinase inhibitors on PUMA protein expression induced by cyclic stretch in cardiomyocytes. (A) Representative Western blots for PUMA protein levels in cardiomyocytes subjected to cyclic stretch in the absence or presence of MAP kinase inhibitors, IFN-γ Ab, siRNA and vehicle (DMSO 0.1 %). CM = conditioned medium. (B) Quantitative analysis of PUMA protein levels. The values from stretched cardiomyocytes have been normalized to matched α-tubulin measurement and then expressed as a ratio of normalized values to protein in control group (n = 4 per group). *P < 0.01 vs. control.Back to article page