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Figure 7 | Journal of Biomedical Science

Figure 7

From: Regulation of PUMA induced by mechanical stress in rat cardiomyocytes

Figure 7

Effect of PUMA on stretch-induced apoptosis in cardiomyocytes. (A) Cardiomyocytes were subjected to cyclic stretch for 18 h, addition of 10 μM atorvastatin, siRNA before stretch or addition of 200 pg/ml IFN-γ alone. Quantification of the apoptotic fractions was performed using FACScan. Cells that stain negative for both Annexin V and PI are alive. Cells that stain positive for Annexin V and negative for PI are undergoing apoptosis. Cells that stain positive for both Annexin V and PI are in the end stage of apoptosis called second apoptosis. (n = 3). *P < 0.05 vs. control. **P < 0.05 vs. stretch 18 h. (B) Representative microscopy images of cardiomyocytes after cyclic stretch for 18 h, addition of atorvastatin, siRNA before stretch or addition of IFN-γ, then stained with a TUNEL kit. Similar results were observed in another two independent experiments. Arrow indicates TUNEL positive cells.

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