Figure 2

Different sensitivity to high extracellular Mg²⁺. Time diaries of currents from different myocytes, i.e. without- and with up-regulated TRPM7-like current at rupture (A and B, respectively), are plotted against time at +80 (ο) and -120 mV (), and external inhibition by 7.2 mM Mg²⁺, 100 μM SpM⁴⁺, and 100 μM Gd³⁺ (periods of exposure are indicated by horizontal bars). Corresponding I/V relations (C, D) obtained at rupture (), after 30 min of dialysis (○) and after external application of 7.2 mM Mg²⁺ (▴), respectively. The arrow and horizontal dashed line indicate current amplitude at rupture. Notice different sensitivity to high Mg_o²⁺. In inserts – corresponding Mg²⁺-sensitive currents. Horizontal dot line - zero current level.