Figure 2

Western blot analysis of neurofilament proteins. Protein levels of NF-H, phosphorylated NF-H, NF-M and phosphorylated NF-M are enhanced in pEGFP-Peripherin cells, especially at 8 days of NGF induction. However, protein level of NF-L is not influenced in pEGFP-Peripherin cells. The EGFP-Peripherin fusion protein is constantly expressed in pEGFP-Peripherin cells, while not in PC12 cells. The protein level of endogenous peripherin is similar between control PC12 cells and pEGFP-Peripherin cells.