Figure 3

Cellular distribution of phosphorylated NFs in PC12 and pEGFP-Peripherin cells. (A and B) Distribution of phosphorylated NF proteins in PC12 cells and pEGFP-Peripherin cells after NGF induction for 8 days examined by immunocytochemical staining with antibodies against phosphorylated NF-H (p-NF-H) (A) or phosphorylated NF-M (p-NF-M) (B). p-NF-H and p-NF-M were detected in cytoplasmic aggregates (arrows) in pEGFP-Peripherin cells. Scale bar = 20 μm.