Figure 7

The activity of calpain, caspase-12, caspase-9, and caspase-3 is increased on 8 day of NGF induction in pEGFP-Peripherin cells compared to PC cells. (A) Western blot analysis showing increased activation of caspase-12 (42 kD), caspase-9 (40 kD and 38 kD), and caspase-3 (19 kD and 17 kD) on later stage of NGF induction in pEGFP-Peripherin cells compared to PC cells. (B-E) Density of the bands for the calpain BDP (B), active caspase-12 (C), active caspase-9 (D), or active caspase-3 (E) relative to the β-actin band. The values are presented as the mean ± SEM for three experiments in each group. *p < 0.05, **p < 0.01 vs. control PC12 cells.