Figure 5

Blockage of daidzein-induced neuritogenesis by kinase inhibitors of PKCδ. (A) DRG neuronal cultures were treated for 24 h with (a) DMSO, (b) 30 μM daidzein or (d) 2 μM PKCδ inhibitor rotterlin. Inhibitor assay was conducted by pretreatment of DRG neuronal cultures for 30 min with (c) 2 μM rottlerin, followed by 30 μM daidzein for 24 h. The neurons were fixed and immunostained for NF-L. Scale Bar = 30 μm. Analysis of total neurite length (B) and tip number (C) revealed that rottlerin significantly blocked the neuritogenesis induced by daidzein. **, p ≤ 0.01 vs DMSO; #, p ≤ 0.05 vs daidzein, ##, p ≤ 0.01 vs daidzein. N = 10.