Figure 7

Activation of ERK by daidzein treatment, and suppression of the ERK activation by both Src and PKCδ inhibitors. Upper panel showed a representative blot from one experiment. Lower panel showed optical densities of the densitometric scans of the pERK bands. (A) DRG neuronal cultures were treated with DMSO, 30 μM daidzein (Dz) for 30 min, 10 μM the Src inhibitor PP2 for 30 min followed by 30 μM daidzein (Dz + PP2) for 30 min, or 10 μM PP2 for 60 min (PP2). The cell homogenate was analyzed for phosphorylated pERK and total ERK. *, p < 0.05 vs DMSO; #, p < 0.05 vs daidzein. n = 4. (B) DRG neuronal cultures were treated with DMSO, 30 μM daidzein (Dz) for 30 min, 2 μM the PKCδ inhibitor rottlerin for 30 min followed by 30 μM daidzein for 30 min (Dz + rott), or 2 μM rottlerin for 60 min (rott). The cell homogenate was analyzed for phosphorylated pERK and total ERK. *, p < 0.05 vs DMSO group; #, p < 0.05 vs daidzein group. n = 4.