Figure 3

Quantitative RT-PCR analysis of hepcidin and HJV mRNA expression in HuH7 cells following IL-6 and TNF-α treatment. A) HuH7 cells were incubated for 24 hours in the presence of vehicle (control) or IL-6 (10 ng/mL). B) HuH7 cells were incubated for 24 hours in the presence of vehicle (control) or TNF-α (20 ng/mL) and quantitative PCR was performed for hepcidin and HJV gene expression. Data are mean ± SEM of 6 samples from 3 separate experiments performed in duplicate. ***p <0.001. (C) HuH7 cells were incubated in the presence of vehicle (control) or TNF-α (20 ng/mL) for 6, 12, 24, and 48 hours. Quantitative PCR for HJV mRNA expression was performed for 6 samples, each run in triplicates, in 2 separate experiments. Data are mean ± SEM . *p <0.05, ***p <0.001.