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Figure 4 | Journal of Biomedical Science

Figure 4

From: Tumour necrosis factor alpha downregulates human hemojuvelin expression via a novel response element within its promoter

Figure 4

Western blot analysis of hepcidin and HJV protein expression in HuH7 cells following IL-6 and TNF-α treatment. A) Cell lysates from control (lanes 1-3) and TNF-α treated (lanes 4-6) cells were analyzed by Western blotting with an antibody to HJV; β-actin was used as loading control. A representative experiment is shown. (B) Cell lysates from control (lanes 1, 3) and IL-6 treated (lanes 2, 4) cells were analyzed by Western blotting with an antibody to hepcidin; β-actin was used as loading control. A representative experiment is shown. Protein molecular weight markers are in kDa.

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Journal of Biomedical Science

ISSN: 1423-0127