Genomic context of the TNFRE in the HJV promoter; and luciferase reporter activity of HJVP1.2-luc transfected HuH7 cells following TNF-α treatment. A) The consensus TNF-α RE (underlined) lies 277 nucleotides upstream of the transcription start site (bold italic). (B) HuH7 cells were transfected with HJVP1.2-luc and incubated for 24 hours with TNF-α (20 ng/ml). Luciferase expression in both treated and untreated cells were normalized to β-galactosidase expression. Data are mean ± SEM, n = 12 from 3 separate experiments; **p <0.0001.