Luciferase reporter activity of HJVP1.2-luc and mt HJVP1.2-luc transfected HuH7 cells following TNF-α treatment. HuH7 cells were transfected with HJVP1.2-luc or mtHJVP1.2-luc to verify that the identified TNFRE is functional. Transfected HuH7 cells were incubated for 24 hours in the presence of TNF-α (20 ng/ml). Data are mean ± SEM, n = 12 of 3 independent experiments. ***p <0.0001.