Proteolytic cleavage of BCL10GFP by MALT1. (A) Lysates of HEK293T cells transfected with BCL10 or BCL10GFP in the absence (-) or the presence (+) of MALT1 as indicated were immunoprecipitated using anti-BCL10 antibody and treated with or without calf intestine alkaline phosphatase (CIAP), and analyzed by Western blotting with anti-BCL10 antibody. filled arrow: full length BCL10; unfilled arrow: truncated BCL10 (B) 2×105 HEK293T cells were transfected with 0.25 μg of pRc/CMVBCL10, pRc/CMVBCL10GFP, or pRc/CMVGFP and 2 μg of pCMV6/XL5 or pCMV6/XL5/MALT1. Western blot analysis of total cell lysates was carried out using anti-BCL10, anti-GFP and anti-MALT1antibodies. B: BCL10; P-B: phosporylated BCL10; BG: BCL10GFP; P-BG: Phosphorylated BCL10GFP.