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Figure 1 | Journal of Biomedical Science

Figure 1

From: BCL10GFP fusion protein as a substrate for analysis of determinants required for Mucosa-Associated Lymphoid Tissue 1 (MALT1)-mediated cleavage

Figure 1

Proteolytic cleavage of BCL10GFP by MALT1. (A) Lysates of HEK293T cells transfected with BCL10 or BCL10GFP in the absence (-) or the presence (+) of MALT1 as indicated were immunoprecipitated using anti-BCL10 antibody and treated with or without calf intestine alkaline phosphatase (CIAP), and analyzed by Western blotting with anti-BCL10 antibody. filled arrow: full length BCL10; unfilled arrow: truncated BCL10 (B) 2×105 HEK293T cells were transfected with 0.25 μg of pRc/CMVBCL10, pRc/CMVBCL10GFP, or pRc/CMVGFP and 2 μg of pCMV6/XL5 or pCMV6/XL5/MALT1. Western blot analysis of total cell lysates was carried out using anti-BCL10, anti-GFP and anti-MALT1antibodies. B: BCL10; P-B: phosporylated BCL10; BG: BCL10GFP; P-BG: Phosphorylated BCL10GFP.

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