Identification of the determinants on BCL10GFP essential for MALT1-induced cleavage. (A) (B) (C) (D) Western blot analysis of lysates from cells transfected with various constructs indicated in the absence (-) or the presence (+) of MALT1 using anti-BCL10, anti-MALT1 antibodies. (E) Left: Western blot analysis of lysates from HEK293T cells transfected with BCL10GFP or BCL10L225RGFP in the absence (-) or the presence (+) of MALT1. Right: Lysates of cells transfected with BCL10, BCL10L225K or BCL10L225R in the absence (-) or the presence (+) of MALT1 were treated with alkaline phosphatase and analyzed by western blot analysis. filled arrow: full length BCL10; unfilled arrow: truncated BCL10; half-filled arrow: cryptically processed BCL10.