Figure 5

The link between IKK and Akt in zerumbone-induced apoptosis . (A) Cells were transfected with pcDNA or IKKα forκ 48 h. After transfection, cells were treated with vehicle or 50 μM zerumbone for another 1 h. The phosphorylation of Akt was then determined by immunoblotting. Each column represents the mean ± S.E.M. of at least 3 independent experiments. *p < 0.05, compared with the group trasfected with pcDNA, in the presence of zerumbone. (B) GBM cells then were transfected with WT Akt for 48 h. Then the cells were treated with zerumbone for 1 h and the phosphorylation of IKKα and IKKβwas measured by immunoblotting. There was no significant difference of phosphorylation of IKKα and IKKβ between cells transfected with empty vector or with WT Akt before treatment with zerumbone.