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Figure 6 | Journal of Biomedical Science

Figure 6

From: Zerumbone suppresses IKKα, Akt, and FOXO1 activation, resulting in apoptosis of GBM 8401 cells

Figure 6

Zerumbone- induced FOXO1 dephosphorylation in GBM cells . (A) Cells were treated with 50 μM zerumbone for indicated time intervals. FOXO1 phosphorylation status was then evaluated by immunoblotting. Each column represents the mean ± S.E.M. of at least 3 independent experiments. *p <0.05, compared with the control group. (B) Cells were transfected with pcDNA or WT IKK for 48 h. After transfection, cells were treated with vehicle or 50 μM zerumbone for 1 h. The phosphorylation status of FOXO1 was then determined by immunoblotting. Each column represents the mean ± S.E.M. of at least three independent experiments. *p < 0.05, compared with the group tranfected with pcDNA, in the presence of zerumbone. (C) Cells were transfected with empty vector (mock) and WT Akt. Then cells were treated with vehicle or 50 μM zerumbone and FOXO1 phosphorylation level was measured by immunoblotting. Each column represents the mean ± S.E.M. of at least 3 independent experiments. *p < 0.05, compared with empty vector, in the presence of zerumbone.

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