Cell cycle arrests by reversine. (A) OSCC cells were treated with DMSO or different concentrations of reversine (1, 5, 10 μM) for 12, 24 and 48 h. Cells were harvested, stained with PI and applied for flow cytometry analysis. The data were analyzed from three independent experiments. (B) As in (A), cells were treated with either different doses of reversine at 24 h or 5 μM reversine at indicated time points. The percentage of cells in each phase of the cell cycle was demonstrated. (C) OSCC cells treated with DMSO or 5 μM reversine were harvested and analyzed for cell cycle-dependent proteins. The GAPDH protein was used as the loading control.