Figure 2

Allele-specific expression of B. t. indicus x B. t. taurus F1 concepti. Shown are two examples of the RFLP assay used to distinguish parent-specific gene expression in B. t. indicus x B. t. taurus F1concepti. DNA sequence polymorphisms between B. t. indicus and B. t. taurus were used as a diagnostic test to identify the parental allele origin of the transcript. A. KCNQ1OT1 (paternally-expressed gene). Lanes = 1: B. t. taurus liver; 2: B. t. indicus kidney, 3: B. t. indicus fat; 4: F1B heart, 5: F1B liver; 6: F1C heart. B. H19 (maternally-expressed gene). Lanes = 1: B. t. taurus muscle; 2: B. t. indicus fat; 3: F1A heart. M = maternal allele, P = paternal allele.