Differential methylation at the KvDMR1 in bovids . Top. Part of KCNQ1 10th intron is drawn to scale and depicted in light purple. Arrow depicts direction of KCNQ1OT1’s transcription. The region amplified by the bisulfite specific primers is represented by a yellow box. Bottom. Shown is an example of bisulfite sequence data from an F1 individual. The bisulfite converted DNA was amplified and cloned prior to sequencing. Each line of circles represents individual alleles. Open circles represent unmethylated CpGs and closed circles represent methylated CpGs. Female symbol = maternal alleles, male symbol = paternal alleles. The position of the SNP used to differentiate between B. t. indicus and B. t. taurus alleles is shown by arrows. The insertion/deletion “GCG” SNP (Table 2) results in an additional CpG site on the paternal alleles compared to maternal alleles.