Table 1 DNA sequence polymorphisms used to ascertain allele-specific expression and methylation
Gene/ICR Symbol | Type of assay | Maternal (B.t. taurus) | Paternal (B.t. indicus) | Location of primers within the gene | NCBI accession # (based on Btau-4.2) | PM location in reference Btau 4.2 |
|---|---|---|---|---|---|---|
H19 | gene expression | C | T | Exon 2–5 | NR_003958.2 | 1831 |
KCNQ1OT1 | gene expression | A | G | Exon 1 (close to the start of transaction) | NW_001494547.3 | 3146321 |
CDKN1C | gene expression | C | T | Exon 2–4 | NW_001494547.3 | 2955801 |
PLAGL1 | gene expression | T | G | Exon 6 | NM_001103289.1 | 867 |
H19/IGF ICR | DNA methylation | A | G | N/A | NW_001494547.3 | 3724402 |
KvDMR1 | DNA methylation | G, A, A in/del between GA, C** | C**, G, G, in/del between GA “GCG”, G | N/A | NW_001494547.3 | 3134118, 3134110, 3134095, 3134087-3134086, 3134072 |
CDKN1C DMR | DNA methylation | N/A | N/A | Exon 1-Intron 2 | NW_001494547.3 | N/A |