Table 3 Restriction enzymes used to determine allele-specific expression of imprinted genes
Gene Symbol | Expressed Allele | Restriction enzyme | Digested B t. taurus (bp) | Digested B. t. indicus (bp) | PAGE Details |
|---|---|---|---|---|---|
H19 | Maternal | BsiHKAI | 609,143 | 609,35,108 | 18% |
KCNQ1OT1 | Paternal | Hinfl | 457,32,13 | 268,189,32,13 | 7% |
CDKN1C | Maternal | Avall | 494,251 | 361,251,133 | 10% |
PLAGL1 | Paternal | Mlul | 834 | 387,447 | 10% |