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Table 3 Restriction enzymes used to determine allele-specific expression of imprinted genes

From: Expression of KCNQ1OT1, CDKN1C, H19, and PLAGL1 and the methylation patterns at the KvDMR1 and H19/IGF2 imprinting control regions is conserved between human and bovine

Gene Symbol Expressed Allele Restriction enzyme Digested B t. taurus (bp) Digested B. t. indicus (bp) PAGE Details
H19 Maternal BsiHKAI 609,143 609,35,108 18%
KCNQ1OT1 Paternal Hinfl 457,32,13 268,189,32,13 7%
CDKN1C Maternal Avall 494,251 361,251,133 10%
PLAGL1 Paternal Mlul 834 387,447 10%
  1. PAGE= Polyacrylamide gel eclectrophoresis, bp= base pairs.