Hemoglobin analysis. Hemoglobin levels in lung tissue homogenate fluid, plasma, and whole blood from PA- and LT-treated rats were determined. The hemoglobin level of whole blood from the vehicle group was set at 100% for the normalization of the data. The vehicle (1/4 diluted) data indicate the reference point of whole blood samples of vehicle-treated group that were diluted to 25% by the addition of phosphate buffered saline. Data were obtained from 3 experiments with 2 replicates (34n = 6). ** p < 0.001, compared to the vehicle lung tissue fluid group.