Figure 3

Co-administration of curdlan decreases the number of epidermal LC in draining LNs at 96 hours post-epicutaneous sensitization. (A) Groups of BALB/c mice were sensitized by patch application with OVA plus curdlan or OVA alone. 24 and 96 hours after patch application, draining LNs were obtained and prepared for positive selection for DCs. Intracellular staining for langerin and surface staining for CD103 were performed. Langerin⁺ CD103^- cells represent epidermal LCs. The results were shown as mean ± SD of pooled data from four independent experiments. (B) 96 hours after patch application, intracellular staining for langerin and surface staining for CD103 and various costimulatory molecules were performed. Langerin⁺ CD103^- cells were gated. Expression levels of CD86 and CD40 were shown. OVA group (gray line), OVA plus curdlan group (black line), isotype control (gray dotted line).