Figure 4

Co-administration of curdlan with OVA influences the cytokine expression profiles in patched skin. Groups of BALB/c mice were sensitized by patch application with OVA plus curdlan or OVA alone. Patched skins were excised 2, 4 and 24 hours after patch application. Total RNA extract, cDNA preparation and quantitative real-time PCR for various cytokines were performed. The relative cytokine mRNA expression of each group was normalized to its β-actin expression. Results are shown as means and standard deviations of pooled data from four independent experiments.