Figure 5

Presence of curdlan in in vitro culture decreases the IL-1α and IL-1β expressions of primary keratinocytes. Skin was obtained from naïve BALB/c mice and prepared into epidermal cell suspension. Keratinocytes were purified by negative selection with MHC class II bead. In vitro culture of primary keratinocytes in the absence or presence of curdlan (50 μg/ml) were performed. Keratinocytes were harvested 0.5, 2, 4 and 24 hours after the start of culture. Measurement of various cytokines were performed similar to Figure 4.