Figure 6

Co-administration of curdlan decreases the proliferation and IL-4 production of specific T cells primed by epidermal LCs. CFSE-labeled CD4 T cell from DO.11.10 mice were intravenously transferred into groups of BALB/c mice three days after they received patch application with OVA plus curdlan or OVA alone. Draining LNs were obtained three days after transfer. (A) After staining with CD4, flow cytometric analysis was performed. CD4+ CFSE+ cells were gated. (B) Pooled results of percentages of divided, labeled CD4 T cells of individual mouse from three independent experiments (n=12) were shown. (C) With intracellular staining for IL-4, pooled results of numbers of IL-4-producing, labeled CD4 T cells of individual mouse from two independent experiments were shown (n=8).