Figure 2

Reactivity of monoclonal antibody 11E12 (A) and antiserum of the mouse immunized with 1–2 nm Aβ1-42 oligomers (B) in Western blot. C – Coomasie blue-stained gel. Lane 1, recombinant fused protein Trx-Aβ1-40; lane 2, E. coli DH5α cells lysate; lane 3, HeLa cells lysate. Lane M, prestained protein molecular weight marker: 130, 100, 70, 55, 40, 25, 15 kDa (Fermentas/Thermo Scientific, Vilnius, Lithuania). The position of recombinant fused protein Trx-Aβ1-40 (molecular weight in a range of 16–20 kDa) is indicated by a dot.