Effect of miR-494 on hypoxia-induced apoptosis was determined with Annexin V-FITC/PI binding staining by flow cytometry. (A) Flow cytometry results with Annexin V-TITC/PI staining. Cells were transfected with miR-494 mimic or miR-negative control as described above. After hypoxia for 8 hours (Top) or 16 hours (Bottom), cells were harvested and then apoptosis was analyzed with an Annexin V-FITC Apoptosis Detection Kit by flow cytometry. Cells were classified as healthy cells (Annexin V−, PI−), early apoptotic cells (Annexin V+, PI−), late apoptotic cells (Annexin V+, PI+), and damaged cells (Annexin V−, PI+). (B) The ratio of apoptosis among different experimental groups. Apoptosis ratio was early apoptosis percentage plus late apoptosis percentage. Control indicated miR-negative control. The data were presented as the means ± SD. Columns, mean of three independent experiments; bars, SD; *p < 0.05.