The expression level of SAP6 is related to its function. (A) The Δsap6 strains display growth defect in media with cell wall attack components. C. albicans strains were spotted on solid medium of YPD or YPD containing Congo red to verify the cell wall integrity of Candida cells. (B) Detection of SAP6 expression by reverse transcription-PCR analysis. The YPD cultured or co-cultured cells were harvested after 30 min incubation at 25°C or 37°C, respectively. Total RNA was isolated and subjected to reverse transcription and PCR analysis.