Figure 7

Reversibility of honokiol effects on calcium signaling. Carbamylcholine (10 μmol/l) was added at the point indicated by the first arrow. Honokiol (3, 10 or 30 μmol/l, as indicated) was added at the time indicated by the second arrow. The cells were washed 3 times over a 2 min period with fresh media, beginning at the time indicated by the third arrow. The cells were again stimulated with carbamylcholine (10 μmol/l) at the time indicated by the fourth arrow. The data is the average from 19-22 cells from a representative experiment performed 3 times with essentially similar results.