Figure 8

Influence of honokiol on ligand binding to muscarinic receptors in rat neocortical membranes. A: The binding of [³H]MS was determined in the absence) open circles) and presence (closed circles) of 10 μmol/l honokiol. Lines are drawn according to nonlinear regression to a model incorporating a single population of independent binding sites that revealed the following binding parameters in the absence and presence of honokiol, respectively: Bmax = 1.3 ± 0.2 and 1.2 ± 0.2 pmol/mg; KD = 0.40 ± 0.03 and 0.35 ± 0.5 nmol/l; N = 3). B: Influence of honokiol on agonist binding to neural muscarinic receptors revealed by the ability of carbamylcholine to inhibit the specific binding of 0.2 nmol/l [³H]MS.