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Table 1 List of primers used in the present study to amplify genes

From: HLA class II SNP interactions and the association with type 1 diabetes mellitus in Bengali speaking patients of Eastern India

Designation Chromosome location Sequence Annealing temperature Product size(bp)
HLA-DPA1* 6p21.3 (Exon2) F-5’ GCGGACCATGTGTGTCAACTTAT 3’ 55°C 210
R-5’ GCCTGAGTGTGGTTGGAACG 3’
HLA-DPB1* 6p21.3 (Exon2) F-5’ GAGAGTGGCGCCTCCGCTCAT 3’ 63°C 327
R-5’ GCCGGCCCAAAGCCCTCACTC 3’
HLA-DQA1 6p21.3$ (Exon1) F-5’ CAAACTCTTCAGCTAGTAAC 3’ 58°C 262
R-5’ CATGCACTCACCCACAAT 3’
6p21.3* (Exon2) F-5’ ATGGTGTAAACTTGTACCAGT 3’ 55°C 229
R-5’ TTGGTAGCAGCGGTAGAGTTG 3’
  6p21.3$ (Exon3) F-5’ AGGTTCCTGAGGTCACAGTGTTT 3’ 54°C 328
R-5’ CTTGACAGACAAGAAAGCATC 3’
HLA-DQB1* 6p21.3(Exon2) F-5’ CATGTGCTACTTCACCAACGG 3’ 58°C 211
R-5’ CTGGTAGTTGTGTCTGCACAC 3’
HLA-DRB1* 6p21.3(Exon2) F-5’ CCCCACAGCACGTTTCTTG 3’ 60°C 274
R-5’ CCGCTGCACTGTGAAGCTCT 3’
  1. *(Tanaka et al. 1999), $(Cordovado et al. 2005).