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Table 1 Primer pair used for real-time (RT) quantitative PCR

From: Pentoxifylline and the proteasome inhibitor MG132 induce apoptosis in human leukemia U937 cells through a decrease in the expression of Bcl-2 and Bcl-XL and phosphorylation of p65

Gene Primer pair sequences Gen bank accession No.
BAK 5CGC TTC GTG GTC GAC TTC AT 3 NM001188
  5AGA AGG CAA AGA CTT CGC TTA 3  
BAX 5TTT GCT TCA GGG TTT CAT CC 3 NM138764
  5CAG TTG AAG TTG CCG TCA GA 3  
DIABLO 5TGA CTT CAA AAC ACC AAG AGT A3 NM019887
  5TTT CTG ACG GAG CTC TTC TA 3  
DR4 5CTC GCT GTC CAC TTT CGT CTCT3 NM003844
  5GTC AAA GGG CAC GAT GTT3  
FAS 5TGA ACA TGG AAT CAT CAA GGA3 NM000043
  5CAA AGC CTT TAA CTT GAC TT3  
BCL-XL 5GCA GGC GAC GAG TTT GAA CT 3 NM138578
  5GTG TCT GGT CAT TTC CGA CTG A 3  
MCL-1 5CAC GAG ACG GTC TTC CAA GGA TGC T 3 NM021960
  5CTA GGT TGC TAG GGT GCA ACT CTA GGA 3  
SURVIVIN 5TGA GCT GCA GGT TCC TTA TCT G 3 NM001168
  5GAA TGG CTT TGT GCT TAG TTT T 3  
IkBa 5GGA TAC CTG GAG GAT CAG ATT A 3 NM001278
  5CCA CCT TAG GGA GTA GTA GAT CAA T 3  
P65 5GCA GGC TCC TGT GCG TGT CT 3 NM02975
  5GGT GCT CAG GGA TGA CGT AAA G 3  
RPL32 5GCA TTG ACA ACA GGG TTC GTA G 3 NM000994
  5ATT TAA ACA GAA AAC GTG CAC A 3  
  1. Oligonucleotides were designed using the Oligo v.6 software. Gene sequences were obtained from the GenBank Nucleotide Database of the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov).