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Table 1 Primer pair used for real-time (RT) quantitative PCR

From: Pentoxifylline and the proteasome inhibitor MG132 induce apoptosis in human leukemia U937 cells through a decrease in the expression of Bcl-2 and Bcl-XL and phosphorylation of p65

Gene

Primer pair sequences

Gen bank accession No.

BAK

5CGC TTC GTG GTC GAC TTC AT 3

NM001188

 

5AGA AGG CAA AGA CTT CGC TTA 3

 

BAX

5TTT GCT TCA GGG TTT CAT CC 3

NM138764

 

5CAG TTG AAG TTG CCG TCA GA 3

 

DIABLO

5TGA CTT CAA AAC ACC AAG AGT A3

NM019887

 

5TTT CTG ACG GAG CTC TTC TA 3

 

DR4

5CTC GCT GTC CAC TTT CGT CTCT3

NM003844

 

5GTC AAA GGG CAC GAT GTT3

 

FAS

5TGA ACA TGG AAT CAT CAA GGA3

NM000043

 

5CAA AGC CTT TAA CTT GAC TT3

 

BCL-XL

5GCA GGC GAC GAG TTT GAA CT 3

NM138578

 

5GTG TCT GGT CAT TTC CGA CTG A 3

 

MCL-1

5CAC GAG ACG GTC TTC CAA GGA TGC T 3

NM021960

 

5CTA GGT TGC TAG GGT GCA ACT CTA GGA 3

 

SURVIVIN

5TGA GCT GCA GGT TCC TTA TCT G 3

NM001168

 

5GAA TGG CTT TGT GCT TAG TTT T 3

 

IkBa

5GGA TAC CTG GAG GAT CAG ATT A 3

NM001278

 

5CCA CCT TAG GGA GTA GTA GAT CAA T 3

 

P65

5GCA GGC TCC TGT GCG TGT CT 3

NM02975

 

5GGT GCT CAG GGA TGA CGT AAA G 3

 

RPL32

5GCA TTG ACA ACA GGG TTC GTA G 3

NM000994

 

5ATT TAA ACA GAA AAC GTG CAC A 3

 
  1. Oligonucleotides were designed using the Oligo v.6 software. Gene sequences were obtained from the GenBank Nucleotide Database of the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov).