A recombinant scFv-FasLext as a targeting cytotoxic agent against human Jurkat-Ras cancer

Table 1 IFN- γ treatment enhances the sensitivity of cancer cells to L6scFv- FasL_extmediated apoptosis

Target cells	% Specific killing of 10⁴cells/well by L6scFv-FasL_ext(μl/well)
	30 μl	10 μl	3 μl	1 μl	0.3 μl	0.1 μl
HeLa	84	80	64	32	4	3
IFN-γ-HeLa	87	85	62	60	46	24
468	58	53	41	11	−5	−3
IFN-γ-468	83	81	79	69	35	17
DKBK3	21	13	11	13	10	11
IFN-γ-DKBK3	78	66	44	31	17	7

Breast cancer cells were sensitized by IFN-γ in vitro. Under the cytotoxicity assay condition, IFN-γ (100 ng/ml) enhanced 3-10 folds of the target susceptibility to L6scFv-FasL_ext.

ISSN: 1423-0127