Figure 1

Effects of HCT on cell viability and morphological changes in human lung cancer A549 cells. (A) The A549 cells were treated with HCT (0, 125, 250 and 500 μg/ml) for 24 and 48 h, the viable cells were determined by MTT assay as described in Materials and Methods. The experiments were performed in triplicate (n = 3). ***p < 0.001 was considered significantly different in comparison with the control. (B) The cells’ morphological changes were examined and photographed by phase-contrast microscopy.