Figure 6
MPLA as the adjuvant significantly reduced lung viral titer. Mice were vaccinated with S-OIV H1N1 or NIBRG-14 H5N1 vaccines with or without different adjuvants. For the challenge, 106 TCID50 of virus was inoculated into mice nasal cavities. Three days post challenge, mice lungs were homogenized and inoculated into MDCK cells for 48 h. Virus titers were evaluated using a micro-plaque assay. (A) H1N1 vaccination, H1N1 virus challenge, (B) H5N1 vaccination, H5N1 virus challenge, (C) H5N1 vaccination, H1N1 virus challenge, (D) H1N1 vaccination, H5N1 virus challenge. The TCID50 titers of the virus were calculated by the method of Reed and Muench [reference [24]. P < 0.05 indicated the significance the differences in viral titers between two groups of vaccinated mice.