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Table 1 HRM primer sequences

From: Detection of epidermal growth factor receptor mutations in formalin fixed paraffin embedded biopsies in Malaysian non-small cell lung cancer patients

Exon Primer name Sequence Amplicon size
18 EGFR_ex18_F 5′-CATGGTGAGGGCTGAGGTGA-3′ 199 bp
EGFR_ex18_R 5′-CCAGAGG(A*)CTGTGCCAGGGAC-3′
19 EGFR_ex19_F 5′-GTGCATCGCTGGTAACATCCA-3 250 bp
EGFR_ex19_R 5′-AAAGGTGGGCCTGAGGTTCA-3
20a EGFR_ex20a_F 5′-AAGCCACACTGACGTGCCTCT-3′ 121 bp
EGFR_ex20a_R 5′-GCGTGATGAG(G*)TGCACGGT-3′
20b EGFR_ex20b_F 5′-CCTCCACCGTGCA(C*)CTCATC-3′ 146 bp
EGFR_ex20b_R 5′-CCCGTATCTCCCTTCCCTGA-3′
21 EGFR_ex21_F 5′-CCTCACAGCAGGGTCTTCTCTG-3′ 210 bp
EGFR_ex21_R 5′-TGGCTGACCTAAAGCCACCTC-3′