The interaction region of CAD with mLST8. (A) The schematic structure of CAD and the fragments employed. CAD and each fragment are shown with the amino acid numbers. (B) Association of mLST8 with the CAD fragments. HEK293 cells were transfected with the FLAG-mLST8 vector and Myc-tagged CAD expression vector of either the full length or each deletion mutant, and the cell extracts were subjected to immunoprecipitation with the anti-myc antibody. The extracts and immunoprecipitates were analyzed by immunoblot with the anti-myc or anti-FLAG antibody. The positions of CAD and its fragments are indicated by arrowheads. *: a non-specific protein.