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Figure 3 | Journal of Biomedical Science

Figure 3

From: Association of CAD, a multifunctional protein involved in pyrimidine synthesis, with mLST8, a component of the mTOR complexes

Figure 3

Association of the mLST8 point mutants with CAD and mTOR. (A) Association of the mLST8 point mutants with CAD. HEK293 cells were transfected with the myc-CAD vector and the FLAG-tagged expression vector of either the wild type or each point mutant of mLST8 as indicated. The extracts and immunoprecipitates with the anti-FLAG antibody were analyzed by immunoblot with the anti-FLAG and anti-myc antibodies. (B) Association of the mLST8 point mutants with mTOR. HEK293 cells were transfected with the HA-mTOR vector and the FLAG-tagged expression vector of either the wild type or each point mutant of mLST8 as indicated. The immunoprecipitates with the anti-FLAG antibody were analyzed by immunoblot with the anti-HA and anti-FLAG antibodies. *: a non-specific protein.

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