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Figure 5 | Journal of Biomedical Science

Figure 5

From: Proteomic analyses and identification of arginine methylated proteins differentially recognized by autosera from anti-Sm positive SLE patients

Figure 5

CNBP is methylated in vivo and is recognized differentially by Sm1 due to its methylation status. HeLa cells were transfected with pFLAG-CNBP plasmids. The FLAG-tagged proteins were immunoprecipitated by anti-FLAG agarose. Proteins eluted by the FLAG peptide were then analyzed by Western blot analyses with anti-Sm1. The blot was stripped of the interacted antibodies and then probed with a mono- and di-methylarginine-specific antibody 7E6. The same blot was stripped and then re-probed with the anti-FLAG antibody. The internal blank region of the FLAG-CNBP signal of the AdOx- sample detected by anti-FLAG was due to previous stripping.

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Journal of Biomedical Science

ISSN: 1423-0127