Figure 6
Differential detection of CNBP protein by anti-Sm positive SLE sera . (A) HeLa cell extracts prepared from cells that were transfected with the pFLAG-CNBP plasmid and treated with AdOx or not were analyzed by Western blot analyses with an aDMA-specific antibody ASYM24 (left panel) or autosera from SLE patient 18 (right panel) and re-probed with anti-FLAG antibodies. The positions of FLAG-CNBP are indicated by arrows. (B) (His)6-CNBP proteins prepared from E. coli cells co-expressing PRMT1(M-CNBP) or not (CNBP) were analyzed by Western blot analyses. Differential detection of (His)6-CNBP by autosera from patient 5, 18 and Sm1 are shown. Arginine methylation of CNBP was confirmed by the detection with ASYM24. The blot was stripped and re-probed with anti-(His)6 (His-tag) antibodies.