Electrophoretic features of plasma antithrombin in neonate and adult mice during post-natal development. Plasma (1 μL) from mice at different stages of development (the number indicates the age in days) were evaluated in PAGE gels under SDS denaturing (A, C) or native (B) conditions. In order to check for potential biases under native conditions due to antithrombin loading charge, the two last lanes contained 3 μL (3x) of neonate (day +1) plasma and 0.3 μL (1/3) of adult (day +50) plasma. (D) SDS-PAGE of selected fractions obtained after isoelectrofocusing and electroelution of plasma antithrombin from neonate and adult mice [Isoelectric point (pI) values are indicated]. Images are representative of different experiments (A, B, and C: n=3; D: n=2).